• Method

  Sandwich ELISA, HRP/TMB, 12×8-well detachable strips

• Sample type

  Serum, EDTA plasma, citrate plasma, heparın plasma, urine, cell culture supernatant, human and non-human samples

• Sample volume

  10 µl / well

• Assay time

  30 min / 2 h / 1 h / 30 min

• Sensitivity

  0.09 nmol/l (= 6.3 ng/ml)

• Standard range

  0 – 12 nmol/l (= 0 – 836 ng/ml)

• Conversion factor

  1 pmol/l = 69.7 pg/ml (MW: 69.7 kDa)

• Specificity

  Endogenous and recombinant human soluble Neuropilin-1 (isoform 2 and 3).

• Precision

  In-between (n=10): ≤ 10 % CV

  Within-run (n=6): ≤ 11 % CV

• Cross-reactivity

  Due to the high sequence homology between human NPR1 and NPR1 of other species there is potential cross-reactivity with pig and rhesus macaque NPR1 and NPR1 of other species.

  Neuropilin-1 (NRP1) only. The amino acid sequence in the respective binding regions of the antibodies show no homology with NRP2. Thus, cross-reactivity to NRP2 is not expected.

• Validation Data

  See validation data tab for: precision, accuracy, dilution linearity, values for healthy donors, etc

Biomedica

海斯特生物科技有限公司是世界领先的免疫研究试剂制造商，拥有20多年的经验。他们特别关注产品ELISA试剂盒、抗体和蛋白质等等。

With 12 offices in Central and Eastern Europe (CEE) and a team of 250 professionals, Biomedica is a leading distributor of medical devices, in-vitro diagnostics, products for life sciences, Biomedica Immunoassays and Clinical IT. Founded in Vienna more than 35 years ago, we have grown into a multi-national company, still privately held with the main shareholders involved as full-time executives.